Immunogenicity and protective efficacy of a live attenuated H5N1 vaccine in nonhuman primates.
Identifieur interne : 001087 ( Main/Exploration ); précédent : 001086; suivant : 001088Immunogenicity and protective efficacy of a live attenuated H5N1 vaccine in nonhuman primates.
Auteurs : Shufang Fan [République populaire de Chine] ; Yuwei Gao ; Kyoko Shinya ; Chris Kafai Li ; Yanbing Li ; Jianzhong Shi ; Yongping Jiang ; Yongbing Suo ; Tiegang Tong ; Gongxun Zhong ; Jiasheng Song ; Ying Zhang ; Guobin Tian ; Yuntao Guan ; Xiao-Ning Xu ; Zhigao Bu ; Yoshihiro Kawaoka [États-Unis] ; Hualan ChenSource :
- PLoS pathogens [ 1553-7374 ] ; 2009.
Descripteurs français
- KwdFr :
- Administration par voie nasale, Animaux, Anticorps antiviraux (sang), Charge virale, Excrétion virale, Femelle, Infections à Orthomyxoviridae (), Infections à Orthomyxoviridae (virologie), Injections veineuses, Lymphocytes T (immunologie), Macaca mulatta, Réplication virale, Souris, Souris de lignée BALB C, Sous-type H5N1 du virus de la grippe A (immunologie), Sous-type H5N1 du virus de la grippe A (physiologie), Température du corps, Vaccination, Vaccins antigrippaux (immunologie), Vaccins atténués (immunologie), Virus recombinants (immunologie).
- MESH :
- immunologie : Lymphocytes T, Sous-type H5N1 du virus de la grippe A, Vaccins antigrippaux, Vaccins atténués, Virus recombinants.
- physiologie : Sous-type H5N1 du virus de la grippe A.
- sang : Anticorps antiviraux.
- virologie : Infections à Orthomyxoviridae.
- Administration par voie nasale, Animaux, Charge virale, Excrétion virale, Femelle, Infections à Orthomyxoviridae, Injections veineuses, Macaca mulatta, Réplication virale, Souris, Souris de lignée BALB C, Température du corps, Vaccination.
English descriptors
- KwdEn :
- Administration, Intranasal, Animals, Antibodies, Viral (blood), Body Temperature, Female, Influenza A Virus, H5N1 Subtype (immunology), Influenza A Virus, H5N1 Subtype (physiology), Influenza Vaccines (immunology), Injections, Intravenous, Macaca mulatta, Mice, Mice, Inbred BALB C, Orthomyxoviridae Infections (prevention & control), Orthomyxoviridae Infections (virology), Reassortant Viruses (immunology), T-Lymphocytes (immunology), Vaccination, Vaccines, Attenuated (immunology), Viral Load, Virus Replication, Virus Shedding.
- MESH :
- chemical , blood : Antibodies, Viral.
- immunology : Influenza A Virus, H5N1 Subtype, Influenza Vaccines, Reassortant Viruses, T-Lymphocytes, Vaccines, Attenuated.
- physiology : Influenza A Virus, H5N1 Subtype.
- prevention & control : Orthomyxoviridae Infections.
- virology : Orthomyxoviridae Infections.
- Administration, Intranasal, Animals, Body Temperature, Female, Injections, Intravenous, Macaca mulatta, Mice, Mice, Inbred BALB C, Vaccination, Viral Load, Virus Replication, Virus Shedding.
Abstract
The continued spread of highly pathogenic H5N1 influenza viruses among poultry and wild birds, together with the emergence of drug-resistant variants and the possibility of human-to-human transmission, has spurred attempts to develop an effective vaccine. Inactivated subvirion or whole-virion H5N1 vaccines have shown promising immunogenicity in clinical trials, but their ability to elicit protective immunity in unprimed human populations remains unknown. A cold-adapted, live attenuated vaccine with the hemagglutinin (HA) and neuraminidase (NA) genes of an H5N1 virus A/VN/1203/2004 (clade 1) was protective against the pulmonary replication of homologous and heterologous wild-type H5N1 viruses in mice and ferrets. In this study, we used reverse genetics to produce a cold-adapted, live attenuated H5N1 vaccine (AH/AAca) that contains HA and NA genes from a recent H5N1 isolate, A/Anhui/2/05 virus (AH/05) (clade 2.3), and the backbone of the cold-adapted influenza H2N2 A/AnnArbor/6/60 virus (AAca). AH/AAca was attenuated in chickens, mice, and monkeys, and it induced robust neutralizing antibody responses as well as HA-specific CD4+ T cell immune responses in rhesus macaques immunized twice intranasally. Importantly, the vaccinated macaques were fully protected from challenge with either the homologous AH/05 virus or a heterologous H5N1 virus, A/bar-headed goose/Qinghai/3/05 (BHG/05; clade 2.2). These results demonstrate for the first time that a cold-adapted H5N1 vaccine can elicit protective immunity against highly pathogenic H5N1 virus infection in a nonhuman primate model and provide a compelling argument for further testing of double immunization with live attenuated H5N1 vaccines in human trials.
DOI: 10.1371/journal.ppat.1000409
PubMed: 19412338
Affiliations:
- République populaire de Chine, États-Unis
- Wisconsin
- Madison (Wisconsin)
- Université du Wisconsin à Madison
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000236
- to stream PubMed, to step Curation: 000236
- to stream PubMed, to step Checkpoint: 000210
- to stream Ncbi, to step Merge: 000360
- to stream Ncbi, to step Curation: 000360
- to stream Ncbi, to step Checkpoint: 000360
- to stream Main, to step Merge: 001096
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Le document en format XML
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first="Yuwei" last="Gao">Yuwei Gao</name></author><author><name sortKey="Shinya, Kyoko" sort="Shinya, Kyoko" uniqKey="Shinya K" first="Kyoko" last="Shinya">Kyoko Shinya</name></author><author><name sortKey="Li, Chris Kafai" sort="Li, Chris Kafai" uniqKey="Li C" first="Chris Kafai" last="Li">Chris Kafai Li</name></author><author><name sortKey="Li, Yanbing" sort="Li, Yanbing" uniqKey="Li Y" first="Yanbing" last="Li">Yanbing Li</name></author><author><name sortKey="Shi, Jianzhong" sort="Shi, Jianzhong" uniqKey="Shi J" first="Jianzhong" last="Shi">Jianzhong Shi</name></author><author><name sortKey="Jiang, Yongping" sort="Jiang, Yongping" uniqKey="Jiang Y" first="Yongping" last="Jiang">Yongping Jiang</name></author><author><name sortKey="Suo, Yongbing" sort="Suo, Yongbing" uniqKey="Suo Y" first="Yongbing" last="Suo">Yongbing Suo</name></author><author><name sortKey="Tong, Tiegang" sort="Tong, Tiegang" uniqKey="Tong T" first="Tiegang" last="Tong">Tiegang Tong</name></author><author><name sortKey="Zhong, Gongxun" sort="Zhong, Gongxun" uniqKey="Zhong G" first="Gongxun" last="Zhong">Gongxun Zhong</name></author><author><name sortKey="Song, Jiasheng" sort="Song, Jiasheng" uniqKey="Song J" first="Jiasheng" last="Song">Jiasheng Song</name></author><author><name sortKey="Zhang, Ying" sort="Zhang, Ying" uniqKey="Zhang Y" first="Ying" last="Zhang">Ying Zhang</name></author><author><name sortKey="Tian, Guobin" sort="Tian, Guobin" uniqKey="Tian G" first="Guobin" last="Tian">Guobin Tian</name></author><author><name sortKey="Guan, Yuntao" sort="Guan, Yuntao" uniqKey="Guan Y" first="Yuntao" last="Guan">Yuntao Guan</name></author><author><name sortKey="Xu, Xiao Ning" sort="Xu, Xiao Ning" uniqKey="Xu X" first="Xiao-Ning" last="Xu">Xiao-Ning Xu</name></author><author><name sortKey="Bu, Zhigao" sort="Bu, Zhigao" uniqKey="Bu Z" first="Zhigao" last="Bu">Zhigao Bu</name></author><author><name sortKey="Kawaoka, Yoshihiro" sort="Kawaoka, Yoshihiro" uniqKey="Kawaoka Y" first="Yoshihiro" 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China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Animal Influenza Laboratory of the Ministry of Agriculture and National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin</wicri:regionArea><wicri:noRegion>Harbin</wicri:noRegion></affiliation></author><author><name sortKey="Gao, Yuwei" sort="Gao, Yuwei" uniqKey="Gao Y" first="Yuwei" last="Gao">Yuwei Gao</name></author><author><name sortKey="Shinya, Kyoko" sort="Shinya, Kyoko" uniqKey="Shinya K" first="Kyoko" last="Shinya">Kyoko Shinya</name></author><author><name sortKey="Li, Chris Kafai" sort="Li, Chris Kafai" uniqKey="Li C" first="Chris Kafai" last="Li">Chris Kafai Li</name></author><author><name sortKey="Li, Yanbing" sort="Li, Yanbing" uniqKey="Li Y" first="Yanbing" last="Li">Yanbing Li</name></author><author><name sortKey="Shi, Jianzhong" sort="Shi, Jianzhong" uniqKey="Shi J" first="Jianzhong" last="Shi">Jianzhong Shi</name></author><author><name sortKey="Jiang, Yongping" sort="Jiang, Yongping" uniqKey="Jiang Y" first="Yongping" last="Jiang">Yongping Jiang</name></author><author><name sortKey="Suo, Yongbing" sort="Suo, Yongbing" uniqKey="Suo Y" first="Yongbing" last="Suo">Yongbing Suo</name></author><author><name sortKey="Tong, Tiegang" sort="Tong, Tiegang" uniqKey="Tong T" first="Tiegang" last="Tong">Tiegang Tong</name></author><author><name sortKey="Zhong, Gongxun" sort="Zhong, Gongxun" uniqKey="Zhong G" first="Gongxun" last="Zhong">Gongxun Zhong</name></author><author><name sortKey="Song, Jiasheng" sort="Song, Jiasheng" uniqKey="Song J" first="Jiasheng" last="Song">Jiasheng Song</name></author><author><name sortKey="Zhang, Ying" sort="Zhang, Ying" uniqKey="Zhang Y" first="Ying" last="Zhang">Ying Zhang</name></author><author><name sortKey="Tian, Guobin" sort="Tian, Guobin" uniqKey="Tian G" first="Guobin" last="Tian">Guobin Tian</name></author><author><name sortKey="Guan, Yuntao" sort="Guan, Yuntao" uniqKey="Guan Y" first="Yuntao" last="Guan">Yuntao Guan</name></author><author><name sortKey="Xu, Xiao Ning" sort="Xu, Xiao Ning" uniqKey="Xu X" first="Xiao-Ning" last="Xu">Xiao-Ning Xu</name></author><author><name sortKey="Bu, Zhigao" sort="Bu, Zhigao" uniqKey="Bu Z" first="Zhigao" last="Bu">Zhigao Bu</name></author><author><name sortKey="Kawaoka, Yoshihiro" sort="Kawaoka, Yoshihiro" uniqKey="Kawaoka Y" first="Yoshihiro" last="Kawaoka">Yoshihiro Kawaoka</name><affiliation><country>États-Unis</country><placeName><settlement type="city">Madison (Wisconsin)</settlement><region type="state">Wisconsin</region></placeName><orgName type="university" n="3">Université du Wisconsin à Madison</orgName></affiliation></author><author><name sortKey="Chen, Hualan" sort="Chen, Hualan" uniqKey="Chen H" first="Hualan" last="Chen">Hualan Chen</name></author></analytic><series><title level="j">PLoS pathogens</title><idno type="eISSN">1553-7374</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Administration, Intranasal</term><term>Animals</term><term>Antibodies, Viral (blood)</term><term>Body Temperature</term><term>Female</term><term>Influenza A Virus, H5N1 Subtype (immunology)</term><term>Influenza A Virus, H5N1 Subtype (physiology)</term><term>Influenza Vaccines (immunology)</term><term>Injections, Intravenous</term><term>Macaca mulatta</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Orthomyxoviridae Infections (prevention & control)</term><term>Orthomyxoviridae Infections (virology)</term><term>Reassortant Viruses (immunology)</term><term>T-Lymphocytes (immunology)</term><term>Vaccination</term><term>Vaccines, Attenuated (immunology)</term><term>Viral Load</term><term>Virus Replication</term><term>Virus Shedding</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Administration par voie nasale</term><term>Animaux</term><term>Anticorps antiviraux (sang)</term><term>Charge virale</term><term>Excrétion virale</term><term>Femelle</term><term>Infections à Orthomyxoviridae ()</term><term>Infections à Orthomyxoviridae (virologie)</term><term>Injections veineuses</term><term>Lymphocytes T (immunologie)</term><term>Macaca mulatta</term><term>Réplication virale</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Sous-type H5N1 du virus de la grippe A (immunologie)</term><term>Sous-type H5N1 du virus de la grippe A (physiologie)</term><term>Température du corps</term><term>Vaccination</term><term>Vaccins antigrippaux (immunologie)</term><term>Vaccins atténués (immunologie)</term><term>Virus recombinants (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Lymphocytes T</term><term>Sous-type H5N1 du virus de la grippe A</term><term>Vaccins antigrippaux</term><term>Vaccins atténués</term><term>Virus recombinants</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A Virus, H5N1 Subtype</term><term>Influenza Vaccines</term><term>Reassortant Viruses</term><term>T-Lymphocytes</term><term>Vaccines, Attenuated</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Sous-type H5N1 du virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Influenza A Virus, H5N1 Subtype</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Orthomyxoviridae Infections</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à Orthomyxoviridae</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Orthomyxoviridae Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Administration, Intranasal</term><term>Animals</term><term>Body Temperature</term><term>Female</term><term>Injections, Intravenous</term><term>Macaca mulatta</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Vaccination</term><term>Viral Load</term><term>Virus Replication</term><term>Virus Shedding</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Administration par voie nasale</term><term>Animaux</term><term>Charge virale</term><term>Excrétion virale</term><term>Femelle</term><term>Infections à Orthomyxoviridae</term><term>Injections veineuses</term><term>Macaca mulatta</term><term>Réplication virale</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Température du corps</term><term>Vaccination</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The continued spread of highly pathogenic H5N1 influenza viruses among poultry and wild birds, together with the emergence of drug-resistant variants and the possibility of human-to-human transmission, has spurred attempts to develop an effective vaccine. Inactivated subvirion or whole-virion H5N1 vaccines have shown promising immunogenicity in clinical trials, but their ability to elicit protective immunity in unprimed human populations remains unknown. A cold-adapted, live attenuated vaccine with the hemagglutinin (HA) and neuraminidase (NA) genes of an H5N1 virus A/VN/1203/2004 (clade 1) was protective against the pulmonary replication of homologous and heterologous wild-type H5N1 viruses in mice and ferrets. In this study, we used reverse genetics to produce a cold-adapted, live attenuated H5N1 vaccine (AH/AAca) that contains HA and NA genes from a recent H5N1 isolate, A/Anhui/2/05 virus (AH/05) (clade 2.3), and the backbone of the cold-adapted influenza H2N2 A/AnnArbor/6/60 virus (AAca). AH/AAca was attenuated in chickens, mice, and monkeys, and it induced robust neutralizing antibody responses as well as HA-specific CD4+ T cell immune responses in rhesus macaques immunized twice intranasally. Importantly, the vaccinated macaques were fully protected from challenge with either the homologous AH/05 virus or a heterologous H5N1 virus, A/bar-headed goose/Qinghai/3/05 (BHG/05; clade 2.2). These results demonstrate for the first time that a cold-adapted H5N1 vaccine can elicit protective immunity against highly pathogenic H5N1 virus infection in a nonhuman primate model and provide a compelling argument for further testing of double immunization with live attenuated H5N1 vaccines in human trials.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Wisconsin</li></region><settlement><li>Madison (Wisconsin)</li></settlement><orgName><li>Université du Wisconsin à Madison</li></orgName></list><tree><noCountry><name sortKey="Bu, Zhigao" sort="Bu, Zhigao" uniqKey="Bu Z" first="Zhigao" last="Bu">Zhigao Bu</name><name sortKey="Chen, Hualan" sort="Chen, Hualan" uniqKey="Chen H" first="Hualan" last="Chen">Hualan Chen</name><name sortKey="Gao, Yuwei" sort="Gao, Yuwei" uniqKey="Gao Y" first="Yuwei" last="Gao">Yuwei Gao</name><name sortKey="Guan, Yuntao" sort="Guan, Yuntao" uniqKey="Guan Y" first="Yuntao" last="Guan">Yuntao Guan</name><name sortKey="Jiang, Yongping" sort="Jiang, Yongping" uniqKey="Jiang Y" first="Yongping" last="Jiang">Yongping Jiang</name><name sortKey="Li, Chris Kafai" sort="Li, Chris Kafai" uniqKey="Li C" first="Chris Kafai" last="Li">Chris Kafai Li</name><name sortKey="Li, Yanbing" sort="Li, Yanbing" uniqKey="Li Y" first="Yanbing" last="Li">Yanbing Li</name><name sortKey="Shi, Jianzhong" sort="Shi, Jianzhong" uniqKey="Shi J" first="Jianzhong" last="Shi">Jianzhong Shi</name><name sortKey="Shinya, Kyoko" sort="Shinya, Kyoko" uniqKey="Shinya K" first="Kyoko" last="Shinya">Kyoko Shinya</name><name sortKey="Song, Jiasheng" sort="Song, Jiasheng" uniqKey="Song J" first="Jiasheng" last="Song">Jiasheng Song</name><name sortKey="Suo, Yongbing" sort="Suo, Yongbing" uniqKey="Suo Y" first="Yongbing" last="Suo">Yongbing Suo</name><name sortKey="Tian, Guobin" sort="Tian, Guobin" uniqKey="Tian G" first="Guobin" last="Tian">Guobin Tian</name><name sortKey="Tong, Tiegang" sort="Tong, Tiegang" uniqKey="Tong T" first="Tiegang" last="Tong">Tiegang Tong</name><name sortKey="Xu, Xiao Ning" sort="Xu, Xiao Ning" uniqKey="Xu X" first="Xiao-Ning" last="Xu">Xiao-Ning Xu</name><name sortKey="Zhang, Ying" sort="Zhang, Ying" uniqKey="Zhang Y" first="Ying" last="Zhang">Ying Zhang</name><name sortKey="Zhong, Gongxun" sort="Zhong, Gongxun" uniqKey="Zhong G" first="Gongxun" last="Zhong">Gongxun Zhong</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Fan, Shufang" sort="Fan, Shufang" uniqKey="Fan S" first="Shufang" last="Fan">Shufang Fan</name></noRegion></country><country name="États-Unis"><region name="Wisconsin"><name sortKey="Kawaoka, Yoshihiro" sort="Kawaoka, Yoshihiro" uniqKey="Kawaoka Y" first="Yoshihiro" last="Kawaoka">Yoshihiro Kawaoka</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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